pOTTC475 - pAAV c-fos iRFP
(Plasmid #47906)

• Purpose: An AAV vector that expresses iRFP under the c-fos promoter.
• Depositing Lab: Brandon Harvey
• Publication: Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.

Backbone

• Vector backbone: pAAV c-fos eYFP
• Backbone manufacturer: NIDA OTTC
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Infrared fluorescent protein
• Alt name: iRFP
• Species: Rhodopseudomonas palustris
• Insert Size (bp): 951
• GenBank ID: JN247409.1
• Promoter: c-fos

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: c-fos int1 F625 (5'-GGAAGACATAAGCAGTCTCTGACCG )

Resource Information

• A portion of this plasmid was derived from a plasmid made by: the ORF for iRFP was obtained from Addgene (Plasmid 31857). the c-fos promoter was obtained from fosGFP (Barth et al, The Journal of Neuroscience, July 21, 2004•24(29):6466 – 6475).
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that the "N" in the depositor's sequences is legitimately ambiguous in both the Addgene stock and the depositor's original stock. This repeat region may vary in different preps of the plasmid, but it should not affect the plasmid function.