pMXs-hLIN28A
(Plasmid #47902)

• Purpose: Expresses human LIN28A in mammalian cells using retrovirus system.
• Depositing Lab: Shinya Yamanaka
• Publication: Tanabe et al Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):12172-9. doi: 10.1073/pnas.1310291110. Epub 2013 Jun 28.

Backbone

• Vector backbone: pMXs
• Backbone manufacturer: Dr.Toshio Kitamura of the University of Tokyo
• Backbone size w/o insert (bp): 4800
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human LIN28A
• Species: H. sapiens (human)
• Insert Size (bp): 630
• GenBank ID: NM_024674
• Entrez Gene: LIN28A (a.k.a. CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A)
• Promoter: LTR

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: pMXs-S1811
• 3′ sequencing primer: pMXs-AS3200

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.