pRK5-myc-Miro2 A13V
(Plasmid
#47896)
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PurposeExpresses myc tagged Miro2 A13V constitutively active mutant
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 47896 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRK5-myc
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Backbone manufacturerDr. Alan Hall, MRC-LMCB
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Modifications to backboneMyc inserted into EcoRI/PstI site (sites destroyed).
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMiro2 A13V
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Alt nameRHOT2
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Alt nameras homolog family member T2
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Alt nameMiro-2
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SpeciesH. sapiens (human)
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MutationA13V, constitutively active mutant
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GenBank IDAJ517413
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Entrez GeneRHOT2 (a.k.a. ARHT2, C16orf39, MIRO-2, MIRO2, RASL)
- Promoter CMV
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Tag
/ Fusion Protein
- myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK5-myc-Miro2 A13V was a gift from Pontus Aspenström (Addgene plasmid # 47896 ; http://n2t.net/addgene:47896 ; RRID:Addgene_47896) -
For your References section:
The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in mitochondrial trafficking. Fransson S, Ruusala A, Aspenstrom P. Biochem Biophys Res Commun. 2006 Jun 2;344(2):500-10. Epub 2006 Apr 3. 10.1016/j.bbrc.2006.03.163 PubMed 16630562
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/11/95/730f57fa-00fa-11e3-91f9-003048dd6500.png.940x940_q85_autocrop.png)