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Addgene

pRK5-myc-Miro1 E208K/E328K
(Plasmid #47894)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47894 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5-myc
  • Backbone manufacturer
    Dr. Alan Hall, MRC-LMCB
  • Modifications to backbone
    Myc inserted into EcoRI/PstI site (sites destroyed).
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Miro1 E208K/E328K
  • Alt name
    RHOT1
  • Alt name
    ras homolog family member T1
  • Alt name
    MIRO-1
  • Species
    H. sapiens (human)
  • Mutation
    E208K/E328K, abolishes calcium binding
  • GenBank ID
    AJ517412
  • Entrez Gene
    RHOT1 (a.k.a. ARHT1, MIRO-1, MIRO1)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-myc-Miro1 E208K/E328K was a gift from Pontus Aspenström (Addgene plasmid # 47894 ; http://n2t.net/addgene:47894 ; RRID:Addgene_47894)

  • For your References section:

    The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in mitochondrial trafficking. Fransson S, Ruusala A, Aspenstrom P. Biochem Biophys Res Commun. 2006 Jun 2;344(2):500-10. Epub 2006 Apr 3. 10.1016/j.bbrc.2006.03.163 PubMed 16630562
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