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Addgene

pRK5-myc-Miro1 T18N
(Plasmid #47890)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47890 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5-myc
  • Modifications to backbone
    Myc inserted into EcoRI/PstI site (sites destroyed).
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Miro1 T18N
  • Alt name
    RHOT1
  • Alt name
    ras homolog family member T1
  • Alt name
    MIRO-1
  • Species
    H. sapiens (human)
  • Mutation
    T18N, dominant negative mutant
  • GenBank ID
    AJ517412
  • Entrez Gene
    RHOT1 (a.k.a. ARHT1, MIRO-1, MIRO1)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-myc-Miro1 T18N was a gift from Pontus Aspenström (Addgene plasmid # 47890 ; http://n2t.net/addgene:47890 ; RRID:Addgene_47890)

  • For your References section:

    Atypical Rho GTPases have roles in mitochondrial homeostasis and apoptosis. Fransson A, Ruusala A, Aspenstrom P. J Biol Chem. 2003 Feb 21;278(8):6495-502. Epub 2002 Dec 12. 10.1074/jbc.M208609200 PubMed 12482879
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