pRK5-myc-Miro1 P13V
(Plasmid #47889)

• Purpose: Expresses myc tagged Miro1 P13V constitutively active mutant
• Depositing Lab: Pontus Aspenström
• Publication: Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502. Epub 2002 Dec 12.

Backbone

• Vector backbone: pRK5-myc
• Modifications to backbone: Myc inserted into EcoRI/PstI site (sites destroyed).
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Miro1 P13V
• Alt name: RHOT1
• Alt name: ras homolog family member T1
• Alt name: MIRO-1
• Species: H. sapiens (human)
• Mutation: P13V, constitutively active mutant
• GenBank ID: AJ517412
• Entrez Gene: RHOT1 (a.k.a. ARHT1, MIRO-1, MIRO1)
• Promoter: CMV
• Tag / Fusion Protein:
  • myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (destroyed during cloning)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: SV40pA-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry