pSTBlue-U6-sgRNA(tdTomato)
(Plasmid #47871)

• Purpose: This plasmid contains expression cassette for sgRNA targeting tdTomato.
• Depositing Labs: Erik Sontheimer, James Thomson
• Publication: Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Backbone

• Vector backbone: pstBlue-1
• Backbone manufacturer: novagen
• Backbone size w/o insert (bp): 3851
• Total vector size (bp): 4301
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sgRNA-tdTomato
• Species: Synthetic
• Insert Size (bp): 151
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Supplemental Documents:
  • GeneBank File
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that this plasmid is only compatible with Neisseria meningitidis CRISPR systems.