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PurposeExpresses methyl-CpG binding protein 2 (Rett Syndrome)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47806 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR223
- Backbone size w/o insert (bp) 5005
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Vector typeGateway entry vector
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMethyl-CpG Binding Protein 2 (Rett Syndrome)
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Alt nameMECP2
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SpeciesH. sapiens (human)
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Insert Size (bp)1461
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Entrez GeneMECP2 (a.k.a. AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RS, RTS, RTT)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-MECP2 was a gift from Huda Zoghbi (Addgene plasmid # 47806 ; http://n2t.net/addgene:47806 ; RRID:Addgene_47806) -
For your References section:
Protein interactome reveals converging molecular pathways among autism disorders. Sakai Y, Shaw CA, Dawson BC, Dugas DV, Al-Mohtaseb Z, Hill DE, Zoghbi HY. Sci Transl Med. 2011 Jun 8;3(86):86ra49. doi: 10.1126/scitranslmed.3002166. 10.1126/scitranslmed.3002166 PubMed 21653829
Map uploaded by the depositor.
