RH4-bio
(Plasmid #47779)

• Purpose: Expresses enzymatically monobiotinylated partial RH4 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
• Depositing Lab: Gavin Wright
• Publication: Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Backbone

• Vector backbone: pTT3
• Backbone manufacturer: Yves Durocher, NRC
• Backbone size w/o insert (bp): 6633
• Total vector size (bp): 9999
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Codon-optimised RH4 (partial)
• Alt name: RBP4
• Species: Synthetic; Plasmodium falciparum
• Insert Size (bp): 3366
• Mutation: Exogenous signal peptide of mouse origin, changed Threonine52, Serine120, Threonine156, Threonine181, Serine270, Serine413, Serine427, Serine454, Threonine506, Threonine555, Threonine664Threonine680, Serine740, Threonine854, Threonine966, Serine991, Threonine1003, Threonine1006, Serine1123 and Serine1130 to Alanine residues
• GenBank ID:
• Promoter: CMV
• Tags / Fusion Proteins:
  • rat Cd4 d3+4 (C terminal on backbone)
  • enzymatic biotinylation sequence (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: CMV-fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pTT3 was obtained under an MTA from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry