pMM2-rPMCA2a
(Plasmid
#47762)
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Purposemammalian expression of rat PMCA2a
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Depositing Labs
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47762 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMM2
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Backbone manufacturerAdamo, H.P. et al. (1992) Biochem. J. 285, 791-797
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePMCA2a
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Alt nameATP2B2
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Alt nameATPase, Ca++ transporting, plasma membrane 2
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SpeciesR. norvegicus (rat)
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Entrez GeneAtp2b2 (a.k.a. PMCA2)
- Promoter SV40
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer pMT2-F
- 3′ sequencing primer pMT2-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMM2-rPMCA2a was a gift from John Penniston & Emanuel Strehler (Addgene plasmid # 47762 ; http://n2t.net/addgene:47762 ; RRID:Addgene_47762) -
For your References section:
Plasma membrane Ca2+ pump isoforms 2a and 2b are unusually responsive to calmodulin and Ca2+. Elwess NL, Filoteo AG, Enyedi A, Penniston JT. J Biol Chem. 1997 Jul 18;272(29):17981-6. 10.1074/jbc.272.29.17981 PubMed 9218424