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Addgene

pMM2-hPMCA4bct120
(Plasmid #47760)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47760 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMM2
  • Backbone manufacturer
    Adamo, H.P. et al. (1992) Biochem. J. 285, 791-797
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PMCA4bct120
  • Alt name
    ATP2B4
  • Alt name
    ATPase, Ca++ transporting, plasma membrane 4
  • Alt name
    PMCA4
  • Species
    H. sapiens (human)
  • Mutation
    deletion of C-terminal 120 aa (removes calmodulin binding site)
  • Entrez Gene
    ATP2B4 (a.k.a. ATP2B2, MXRA1, PMCA4, PMCA4b, PMCA4x)
  • Promoter SV40

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer pMT2-F
  • 3′ sequencing primer pMT2-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMM2-hPMCA4bct120 was a gift from John Penniston & Emanuel Strehler (Addgene plasmid # 47760 ; http://n2t.net/addgene:47760 ; RRID:Addgene_47760)

  • For your References section:

    A highly active 120-kDa truncated mutant of the plasma membrane Ca2+ pump. Enyedi A, Verma AK, Filoteo AG, Penniston JT. J Biol Chem. 1993 May 15;268(14):10621-6. PubMed 8387523
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