mCherry-hPMCA2wb
(Plasmid #47752)

• Purpose: mammalian expression of mCherry tagged hPMCA2, wb splice variant
• Depositing Lab: Emanuel Strehler
• Publication: Strehler unpublished plasmids (unpublished)

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Modifications to backbone: EGFP replaced with mCherry as Age/KpnI fragment
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PMCA2w/b
• Alt name: ATPase, Ca++ transporting, plasma membrane 2
• Alt name: ATP2B2
• Species: H. sapiens (human)
• Mutation: wb splice variant
• Entrez Gene: ATP2B2 (a.k.a. DFNA82, PMCA2, PMCA2a, PMCA2i)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: mCherry F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Similar to Addgene plasmid 47586 except EGFP replaced with mCherry.