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Addgene

mCherry-hPMCA2zb
(Plasmid #47750)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47750 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Modifications to backbone
    EGFP replaced with mCherry as Age/KpnI fragment
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PMCA2z/b
  • Alt name
    ATPase, Ca++ transporting, plasma membrane 2
  • Alt name
    ATP2B2
  • Species
    H. sapiens (human)
  • Mutation
    zb splice variant
  • Entrez Gene
    ATP2B2 (a.k.a. DFNA82, PMCA2, PMCA2a, PMCA2i)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer mCherry F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Similar to Addgene plasmid 47584 except EGFP replaced with mCherry.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-hPMCA2zb was a gift from Emanuel Strehler (Addgene plasmid # 47750 ; http://n2t.net/addgene:47750 ; RRID:Addgene_47750)
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