P12p-bio
(Plasmid
#47726)
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PurposeExpresses enzymatically monobiotinylated full-length P12p ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 47726 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTT3
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Backbone manufacturerYves Durocher, NRC
- Backbone size w/o insert (bp) 6633
- Total vector size (bp) 7620
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCodon-optimised P12p
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SpeciesSynthetic; Plasmodium falciparum
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Insert Size (bp)987
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MutationExogenous signal peptide of mouse origin, changed Threonine186, Threonine244 and Threonine248 to Alanine residues
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GenBank ID
- Promoter CMV
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Tags
/ Fusion Proteins
- rat Cd4 d3+4 (C terminal on backbone)
- enzymatic biotinylation sequence (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer CMV-fwd (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypTT3 was obtained under an MTA from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
P12p-bio was a gift from Gavin Wright (Addgene plasmid # 47726 ; http://n2t.net/addgene:47726 ; RRID:Addgene_47726) -
For your References section:
A library of functional recombinant cell surface and secreted Plasmodium falciparum merozoite proteins. Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, Wright GJ. Mol Cell Proteomics. 2013 Sep 16. 10.1074/mcp.O113.028357 PubMed 24043421