MSP1-bio
(Plasmid #47709)

• Purpose: Expresses enzymatically monobiotinylated full-length MSP1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
• Depositing Lab: Gavin Wright
• Publication: Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Backbone

• Vector backbone: pTT3
• Backbone manufacturer: Yves Durocher, NRC
• Backbone size w/o insert (bp): 6633
• Total vector size (bp): 11679
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Codon-optimised MSP1
• Species: Synthetic; Plasmodium falciparum
• Insert Size (bp): 5046
• Mutation: Exogenous signal peptide of mouse origin, changed Serine123, Threonine262, Threonine491, Serine557, Serine628, Serine823, Threonine835, Threonine920, Serine940, Threonine986, Threonine1012, Serine1110, Threonine1217, Serine1609 to Alanine residues
• Entrez Gene: MSP1 (a.k.a. PFI1475w, MSA1, MSP-1, PMMSA, Pf190, merozoite surface antigen 1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • rat Cd4 d3+4 (C terminal on backbone)
  • enzymatic biotinylation sequence (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: CMV fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pTT3 was obtained under an MTA from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry