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pmalc4x_K398R_TEV
(Plasmid #47701)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47701 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmalC4X
  • Backbone manufacturer
    NEB
  • Backbone size w/o insert (bp) 6645
  • Total vector size (bp) 7458
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Olfactomedin-like domain with K398R mutation
  • Alt name
    K398R
  • Alt name
    OLF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    837
  • Mutation
    changed Lysine 398 to Arginine
  • Entrez Gene
    MYOC (a.k.a. GLC1A, GPOA, JOAG, JOAG1, TIGR)
  • Promoter Ptac
  • Tag / Fusion Protein
    • MBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer MBP-F
  • 3′ sequencing primer M13-F20; M13_pUC_Fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Lieberman Lab recommends E.coli Rosetta Gami 2 competent cells for protein expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmalc4x_K398R_TEV was a gift from Raquel Lieberman (Addgene plasmid # 47701 ; http://n2t.net/addgene:47701 ; RRID:Addgene_47701)

  • For your References section:

    The stability of myocilin olfactomedin domain variants provides new insight into glaucoma as a protein misfolding disorder. Burns JN, Turnage KC, Walker CA, Lieberman RL. Biochemistry. 2011 Jul 5;50(26):5824-33. doi: 10.1021/bi200231x. Epub 2011 Jun 9. 10.1021/bi200231x PubMed 21612213
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