12XCSL-d1EGFP
(Plasmid #47684)

• Purpose: Notch reporter
• Depositing Lab: Urban Lendahl
• Publication: Hansson et al Dev Neurosci. 2006;28(1-2):118-27.

Backbone

• Vector backbone: pGA981-6
• Backbone manufacturer: described in Kato, et al. 1997 PMID 9374409
• Vector type: Notch reporter construct

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TP-1 promoter
• Insert Size (bp): 300
• Mutation: hexamerized 50-bp EBNA2 response element of the TP-1 promoter (ERE-TP1)
• Promoter: NA
• Tag / Fusion Protein:
  • d1EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: NA
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was constructed from the pGa981-6 plasmid by replacing the luciferase gene in the original pGA981-6 plasmid with d1EGFP using XhoI/SmaI sites.

The construction of pGA981-6 was described as: "Six copies of this oligonucleotide were cloned in the BamHI site:
5' strand GATCCCGACT CGTGGGAAAA TGGGCGGAAG GGCACCGTGG GAAAATAGTA............
3' strand ............GGCTGA GCACCCTTTT ACCCGCCTTC CCGTGGCACC CTTTTATCAT CTAG"

Please note that Addgene's sequencing results indicate that the first and fourth ERE-TP1 repeats differ from the above oligo sequence at the twelfth nucleotide (G->A). These differences may have occurred during construction of the original pGA981-6 plasmid.