pNCMV 16E6no* delPDZ
(Plasmid #47678)

• Purpose: PDZ-binding motif is deleted
• Depositing Lab: Karl Munger
• Publication: Spangle et al J Virol. 2012 May 2.

Backbone

• Vector backbone: pNCMV
• Backbone size w/o insert (bp): 6550
• Total vector size (bp): 7000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HPV 16 E6no*
• Alt name: HPV 16E6
• Species: H. sapiens (human); HPV
• Insert Size (bp): 435
• Mutation: del first 7aa and last 6aa, V42L*
• GenBank ID: NC_001526
• Entrez Gene: E6 (a.k.a. HpV16gp1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • FLAG (N terminal on backbone)
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: EcoRV (destroyed during cloning)
• 5′ sequencing primer: CMV bam neo 5'
• 3′ sequencing primer: CMV bam neo 3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*The V42L mutation prevents the internal splicing of the DNA so only the "no *" (unspliced form) of 18E6 is produced.