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Addgene

Danio rerio cryabb
(Plasmid #47665)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47665 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-21b(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21(DE3)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Alpha B-crystallin
  • Alt name
    cryabb
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    558
  • GenBank ID
    BC076518.1 DQ113417.1
  • Entrez Gene
    cryabb (a.k.a. cryab2, wu:fe37f08, zgc:91937)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's full plasmid sequencing results found that the plasmid backbone differs from what is reported in the associated publication, however, the change in backbone is not expected to affect the use of the plasmids to produce recombinant protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Danio rerio cryabb was a gift from Mason Posner (Addgene plasmid # 47665 ; http://n2t.net/addgene:47665 ; RRID:Addgene_47665)

  • For your References section:

    Functional validation of hydrophobic adaptation to physiological temperature in the small heat shock protein alphaA-crystallin. Posner M, Kiss AJ, Skiba J, Drossman A, Dolinska MB, Hejtmancik JF, Sergeev YV. PLoS One. 2012;7(3):e34438. doi: 10.1371/journal.pone.0034438. Epub 2012 Mar 29. 10.1371/journal.pone.0034438 PubMed 22479631
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