pCS-PesaR-Dlux
(Plasmid #47653)

• Purpose: PesaR-D promoter in pCS26
• Depositing Lab: Cynthia Collins
• Publication: Shong et al ACS Synth Biol. 2013 Jul 23.

Backbone

• Vector backbone: pCS-PesaRlux
• Backbone size w/o insert (bp): 9360
• Total vector size (bp): 9647
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PesaR-D
• Insert Size (bp): 287
• Mutation: Added an additional esa box upstream of -35 site in PesaR
• Promoter: PesaR-D

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CCAGCTGGCAATTCCGA
• 3′ sequencing primer: AATCATCACTTTCGGGAA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: PesaR-D was generated by commercial DNA synthesis.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PesaR-D (275 bp, indicated as lowercase letters in the partial sequence) was cloned between XhoI and BamHI in the same was as wt PesaR in pCS-PesaRlux. The -35 and -10 sites are uppercase in the partial sequence. Please see Figure S1 (Shong and Collins, ACS Synthetic Biology, 2013) for sequence alignment of PesaR and PesaR-D. See pCS-PesaRlux for full plasmid backbone sequence and plasmid map.