pCS-PluxIlux
(Plasmid #47641)

• Purpose: wt luxI promoter in pCS26
• Depositing Lab: Cynthia Collins
• Publication: Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub 2013 Feb 6.

Backbone

• Vector backbone: pCS-PesaRlux
• Backbone size w/o insert (bp): 9360
• Total vector size (bp): 9517
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PluxI
• Alt name: luxI promoter
• Species: Vibrio fischeri
• Insert Size (bp): 157
• GenBank ID:
• Promoter: PluxI

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CCAGCTGGCAATTCCGA
• 3′ sequencing primer: AATCATCACTTTCGGGAA

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We amplified PluxI from pluxGFPuv (Collins CH et al., 2005, Mol. Microbiol.).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PluxI (145 bp, indicated as lowercase letters in the partial sequence) was cloned into pCS-PesaRlux between XhoI and BamHI sites, replacing PesaR with PluxI. Please see pCS-PesaRlux for full plasmid backbone sequence and plasmid map.