pOTTC337 - pAAV EF1a DIO mCherry
(Plasmid #47636)

• Purpose: An AAV vector that expresses mCherry in a Cre-dependent manner (DIO, double floxed inverted open reading frame).
• Depositing Lab: Brandon Harvey
• Publication: Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.

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Backbone

• Vector backbone: pAAV EF1a DIO
• Backbone manufacturer: Karl Deisseroth
• Backbone size w/o insert (bp): 5637
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry
• Species: Discosoma sp
• Promoter: EF1a

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: EF1a_F1
• 3′ sequencing primer: WPRE_R1

Resource Information

• A portion of this plasmid was derived from a plasmid made by: the ORF for mCherry was obtained from Karl Deisseroth, but is also available from Addgene (Plasmid 26976). the backbone was obtained from Karl Deisseroth, but is also available from Addgene (Plasmid 35507).
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector has a different 5' cloning junction when compared to Karl Deisseroth's Plasmid 20299.