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Danio rerio cryaa T147V
(Plasmid #47628)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47628 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-20b(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3716
  • Total vector size (bp) 4150
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL21(DE3)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Alpha A-crystallin
  • Alt name
    cryaa
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    522
  • Mutation
    Changed Theronine 147 to Valine
  • GenBank ID
    BC164886.1
  • Entrez Gene
    cryaa (a.k.a. wu:fc06h04, zgc:92036)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde I (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Danio rerio cryaa T147V was a gift from Mason Posner (Addgene plasmid # 47628 ; http://n2t.net/addgene:47628 ; RRID:Addgene_47628)

  • For your References section:

    Functional validation of hydrophobic adaptation to physiological temperature in the small heat shock protein alphaA-crystallin. Posner M, Kiss AJ, Skiba J, Drossman A, Dolinska MB, Hejtmancik JF, Sergeev YV. PLoS One. 2012;7(3):e34438. doi: 10.1371/journal.pone.0034438. Epub 2012 Mar 29. 10.1371/journal.pone.0034438 PubMed 22479631
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