Skip to main content
Addgene

CMV/N3IC-HA
(Plasmid #47618)

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47618 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCMX
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Mammalian Expression ; in vitro transcription and translation

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Notch3 Intracellular domain
  • Alt name
    Notch3
  • Alt name
    Notch 3
  • Species
    M. musculus (mouse)
  • Mutation
    codons 1664 (Met) to 2318 (Ala)
  • Entrez Gene
    Notch3 (a.k.a. N3, hpbk)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site XbaI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV/N3IC-HA was a gift from Urban Lendahl (Addgene plasmid # 47618 ; http://n2t.net/addgene:47618 ; RRID:Addgene_47618)
  • For your References section:

    Expression of the Notch 3 intracellular domain in mouse central nervous system progenitor cells is lethal and leads to disturbed neural tube development. Lardelli M, Williams R, Mitsiadis T, Lendahl U. Mech Dev. 1996 Oct;59(2):177-90. 10.1016/0925-4773(96)00589-8 PubMed 8951795