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Addgene

GST-N1IC
(Plasmid #47612)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47612 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4t-3
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Notch1 intracellular domain
  • Alt name
    Notch1 IC
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2700
  • Mutation
    contains amino acids 1753-2531
  • GenBank ID
    AAM28905.1
  • Entrez Gene
    Notch1 (a.k.a. 9930111A19Rik, Mis6, N1, Tan1, lin-12)
  • Promoter tac
  • Tags / Fusion Proteins
    • GST (N terminal on backbone)
    • myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer pGEX5'
  • 3′ sequencing primer pGEX3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Construct also called GST-1111.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-N1IC was a gift from Urban Lendahl (Addgene plasmid # 47612 ; http://n2t.net/addgene:47612 ; RRID:Addgene_47612)

  • For your References section:

    The notch 3 intracellular domain represses notch 1-mediated activation through Hairy/Enhancer of split (HES) promoters. Beatus P, Lundkvist J, Oberg C, Lendahl U. Development. 1999 Sep;126(17):3925-35. PubMed 10433920
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