GST-N1IC
(Plasmid
#47612)
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Purposebacterial expression of GST-myc-Notch1 IC
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 47612 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX-4t-3
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Backbone manufacturerAmersham
- Backbone size w/o insert (bp) 4900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNotch1 intracellular domain
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Alt nameNotch1 IC
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SpeciesM. musculus (mouse)
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Insert Size (bp)2700
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Mutationcontains amino acids 1753-2531
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GenBank IDAAM28905.1
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Entrez GeneNotch1 (a.k.a. 9930111A19Rik, Mis6, N1, Tan1, lin-12)
- Promoter tac
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Tags
/ Fusion Proteins
- GST (N terminal on backbone)
- myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SmaI (destroyed during cloning)
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer pGEX3' (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Construct also called GST-1111.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST-N1IC was a gift from Urban Lendahl (Addgene plasmid # 47612 ; http://n2t.net/addgene:47612 ; RRID:Addgene_47612) -
For your References section:
The notch 3 intracellular domain represses notch 1-mediated activation through Hairy/Enhancer of split (HES) promoters. Beatus P, Lundkvist J, Oberg C, Lendahl U. Development. 1999 Sep;126(17):3925-35. PubMed 10433920
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/25/82/80bb9bf4-faae-11e2-8605-003048dd6500.png.940x940_q85_autocrop.png)