pcDNA3.1-6His-MyoX
(Plasmid
#47607)
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47607 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepcDNA3.1-His
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameMyoX
-
Alt nameMYO10
-
Alt namemyosin X
-
SpeciesH. sapiens (human)
-
GenBank IDAF234532.1
-
Entrez GeneMYO10 (a.k.a. MyoX)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note: The His tag from the backbone is not in-frame with the insert.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3.1-6His-MyoX was a gift from Emanuel Strehler (Addgene plasmid # 47607 ; http://n2t.net/addgene:47607 ; RRID:Addgene_47607) -
For your References section:
Calmodulin-like protein enhances myosin-10 translation. Bennett RD, Strehler EE. Biochem Biophys Res Commun. 2008 May 2;369(2):654-9. doi: 10.1016/j.bbrc.2008.02.056. Epub 2008 Feb 22. 10.1016/j.bbrc.2008.02.056 PubMed 18295593