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pCMV-tag2B-PISP/AIPP1
(Plasmid #47606)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47606 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-tag2
  • Backbone manufacturer
    Stratagene
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PISP
  • Alt name
    PDZD11
  • Alt name
    PDZ domain containing 11
  • Species
    H. sapiens (human)
  • Entrez Gene
    PDZD11 (a.k.a. AIPP1, PDZK11, PISP)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-tag2B-PISP/AIPP1 was a gift from Emanuel Strehler (Addgene plasmid # 47606 ; http://n2t.net/addgene:47606 ; RRID:Addgene_47606)

  • For your References section:

    Characterization of PISP, a novel single-PDZ protein that binds to all plasma membrane Ca2+-ATPase b-splice variants. Goellner GM, DeMarco SJ, Strehler EE. Ann N Y Acad Sci. 2003 Apr;986:461-71. 10.1111/j.1749-6632.2003.tb07230.x PubMed 12763866
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