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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47603 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEYFP
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCaM
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Alt nameCALM3
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Alt nameHGNC:1449
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SpeciesH. sapiens (human)
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Entrez GeneCALM3 (a.k.a. CALM, CAM1, CAM2, CAMB, CPVT6, CaM, CaMIII, HEL-S-72, LQT16, PHKD, PHKD3)
- Promoter CMV
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Tag
/ Fusion Protein
- EYFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site XmaI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EYFP-hCaM was a gift from Emanuel Strehler (Addgene plasmid # 47603 ; http://n2t.net/addgene:47603 ; RRID:Addgene_47603) -
For your References section:
The tumor-sensitive calmodulin-like protein is a specific light chain of human unconventional myosin X. Rogers MS, Strehler EE. J Biol Chem. 2001 Apr 13;276(15):12182-9. Epub 2001 Jan 22. 10.1074/jbc.M010056200 PubMed 11278607
