pGEMEX174-hCALML3
(Plasmid #47600)

• Depositing Lab: Emanuel Strehler
• Publication: Qian et al Protein Sci. 1998 Nov;7(11):2421-30.

Backbone

• Vector backbone: pGEMEX-2
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CALM3
• Alt name: calmodulin-like 3
• Alt name: CLP
• Species: H. sapiens (human)
• Entrez Gene: CALML3 (a.k.a. CLP)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

We observed in preliminary experiments that IPTG added to the growth media did not induce substantial additional CLP expression. In addition, we found that the addition of glucose to the growth medium significantly inhibited CLP expression from this vector. An NdeI site at position 3259 has been abolished.