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Addgene

pKK233-hCALML3
(Plasmid #47599)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47599 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKK233-2
  • Backbone manufacturer
    Pharmacia
  • Backbone size w/o insert (bp) 4580
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CALML3
  • Alt name
    calmodulin like 3
  • Alt name
    CLP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    538
  • Mutation
    contains 538 bp fragment
  • Entrez Gene
    CALML3 (a.k.a. CLP)
  • Promoter trc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer M13pUC-Rev
  • 3′ sequencing primer pBAD-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKK233-hCALML3 was a gift from Emanuel Strehler (Addgene plasmid # 47599 ; http://n2t.net/addgene:47599 ; RRID:Addgene_47599)

  • For your References section:

    Characterization of the human calmodulin-like protein expressed in Escherichia coli. Rhyner JA, Koller M, Durussel-Gerber I, Cox JA, Strehler EE. Biochemistry. 1992 Dec 29;31(51):12826-32. 10.1021/bi00166a017 PubMed 1334432
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