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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47598 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepKK233-2
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Backbone manufacturerPharmacia
- Backbone size w/o insert (bp) 4580
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCaM
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Alt nameCALM3
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Alt nameHGNC:1449
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SpeciesH. sapiens (human)
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Insert Size (bp)489
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Mutationcontains 469 bp fragment (nt 92-559)
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Entrez GeneCALM3 (a.k.a. CALM, CAM1, CAM2, CAMB, CPVT6, CaM, CaMIII, HEL-S-72, LQT16, PHKD, PHKD3)
- Promoter trc
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer M13pUC-Rev
- 3′ sequencing primer pBAD-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKK233-hCaM was a gift from Emanuel Strehler (Addgene plasmid # 47598 ; http://n2t.net/addgene:47598 ; RRID:Addgene_47598) -
For your References section:
Characterization of the human calmodulin-like protein expressed in Escherichia coli. Rhyner JA, Koller M, Durussel-Gerber I, Cox JA, Strehler EE. Biochemistry. 1992 Dec 29;31(51):12826-32. 10.1021/bi00166a017 PubMed 1334432
