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Addgene

EGFP-hPMCA4b delta6
(Plasmid #47590)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47590 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PMCA4bdelta6
  • Alt name
    ATPase, Ca++ transporting, plasma membrane 4
  • Alt name
    ATP2B4
  • Species
    H. sapiens (human)
  • Mutation
    6 C-terminal AA deleted (needed for PDZ binding)
  • Entrez Gene
    ATP2B4 (a.k.a. ATP2B2, MXRA1, PMCA4, PMCA4b, PMCA4x)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

x/b splice variant of human PMCA4

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP-hPMCA4b delta6 was a gift from Emanuel Strehler (Addgene plasmid # 47590 ; http://n2t.net/addgene:47590 ; RRID:Addgene_47590)

  • For your References section:

    PSD-95 mediates membrane clustering of the human plasma membrane Ca2+ pump isoform 4b. Padanyi R, Paszty K, Strehler EE, Enyedi A. Biochim Biophys Acta. 2009 Jun;1793(6):1023-32. doi: 10.1016/j.bbamcr.2008.11.007. Epub 2008 Nov 27. 10.1016/j.bbamcr.2008.11.007 PubMed 19073225
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