EGFP-hPCM2w/b delta6
(Plasmid
#47588)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 47588 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech; modified as described in Rogers et al JBC 2005
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePMCA2w/b delta6
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Alt nameATPase, Ca++ transporting, plasma membrane 2
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Alt nameATP2B2
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SpeciesH. sapiens (human)
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Insert Size (bp)3728
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Mutation6 C-terminal AA deleted (needed for PDZ binding)
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Entrez GeneATP2B2 (a.k.a. DFNA82, PMCA2, PMCA2a, PMCA2i)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP-HA-His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP-hPCM2w/b delta6 was a gift from Emanuel Strehler (Addgene plasmid # 47588 ; http://n2t.net/addgene:47588 ; RRID:Addgene_47588) -
For your References section:
Alternative splicing of the first intracellular loop of plasma membrane Ca2+-ATPase isoform 2 alters its membrane targeting. Chicka MC, Strehler EE. J Biol Chem. 2003 May 16;278(20):18464-70. Epub 2003 Mar 6. 10.1074/jbc.M301482200 PubMed 12624087