EGFP-hPMCA2w/b
(Plasmid #47586)

• Depositing Lab: Emanuel Strehler
• Publication: Chicka et al J Biol Chem. 2003 May 16;278(20):18464-70. Epub 2003 Mar 6.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech; modified as described in Rogers et al JBC 2003
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PMCA2w/b
• Alt name: ATPase, Ca++ transporting, plasma membrane 2
• Alt name: ATP2B2
• Species: H. sapiens (human)
• Insert Size (bp): 3754
• Entrez Gene: ATP2B2 (a.k.a. DFNA82, PMCA2, PMCA2a, PMCA2i)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP-HA-His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacII (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

w/b splice variant of human PMCA2. PMCA2w/b inserted as SacII-XbaI fragment into multiple cloning site but is also flanked by NotI sites. PMCA2 start: 1396; stop: 5125, w-splice: 2303