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Addgene

pMM2-hPMCA2x/b
(Plasmid #47582)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47582 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMM2
  • Backbone manufacturer
    Penniston Lab
  • Backbone size w/o insert (bp) 5145
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PMCA2x/b
  • Alt name
    ATPase, Ca++ transporting, plasma membrane 2
  • Alt name
    ATP2B2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3698
  • Entrez Gene
    ATP2B2 (a.k.a. DFNA82, PMCA2, PMCA2a, PMCA2i)
  • Promoter SV40

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer pMT2-F
  • 3′ sequencing primer pMT2-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

x/b splice variant of human PMCA2. PMCA2 start: 1090; stop: 4728, x-splice: 1997-2038; b-splice: starts at 4417.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMM2-hPMCA2x/b was a gift from Emanuel Strehler (Addgene plasmid # 47582 ; http://n2t.net/addgene:47582 ; RRID:Addgene_47582)

  • For your References section:

    Alternative splicing of the first intracellular loop of plasma membrane Ca2+-ATPase isoform 2 alters its membrane targeting. Chicka MC, Strehler EE. J Biol Chem. 2003 May 16;278(20):18464-70. Epub 2003 Mar 6. 10.1074/jbc.M301482200 PubMed 12624087
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