pJH136
(Plasmid #47554)

• Purpose: pJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.
• Depositing Lab: Ronald Davis
• Publication: Horecka et al Yeast. 2014 Mar;31(3):103-12. doi: 10.1002/yea.2992. Epub 2013 Dec 13.

Backbone

• Vector backbone: pPCR-Script Amp SK(+)
• Backbone manufacturer: Agilent Technologies
• Backbone size w/o insert (bp): 2961
• Total vector size (bp): 4121
• Vector type: PCR template
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: URA3
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1160
• Promoter: Native URA3 promoter, -243 to -1.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SrfI (destroyed during cloning)
• 3′ cloning site: SrfI (destroyed during cloning)
• 5′ sequencing primer: GTTGTAAAACGACGGCCAGT
• 3′ sequencing primer: TCACACAGGAAACAGCTATGA

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Primer sequences (5' to 3'): U2, CGTACGCTGCAGGTCGAC; D2, ATCGATGAATTCGAGCTCG.