pLX302 Luciferase-V5 puro
(Plasmid #47553)

• Purpose: Lentiviral expression of V5-tagged Luciferase in mammalian cells
• Depositing Lab: Kevin Janes
• Publication: Kang et al Sci Signal. 2013 Aug 6;6(287):rs13. doi: 10.1126/scisignal.2003624.

Backbone

• Vector backbone: pLX302
• Backbone manufacturer: Addgene #25896
• Backbone size w/o insert (bp): 9573
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Luciferase
• Species: Firefly
• Insert Size (bp): 1653
• Mutation: None
• Promoter: CMV
• Tag / Fusion Protein:
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene #25894
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry