pDD162 (Peft-3::Cas9 + Empty sgRNA) Citations (112)
Originally described in: Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination.Dickinson DJ, Ward JD, Reiner DJ, Goldstein B Nat Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641. PubMed Journal
Articles Citing pDD162 (Peft-3::Cas9 + Empty sgRNA)
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| Conditional knockouts generated by engineered CRISPR-Cas9 endonuclease reveal the roles of coronin in C. elegans neural development. Shen Z, Zhang X, Chai Y, Zhu Z, Yi P, Feng G, Li W, Ou G. Dev Cell. 2014 Sep 8;30(5):625-36. doi: 10.1016/j.devcel.2014.07.017. Epub 2014 Aug 21. PubMed |
CRISPR/Cas9 Genome Editing in Caenorhabditis elegans: Evaluation of Templates for Homology-Mediated Repair and Knock-Ins by Homology-Independent DNA Repair.
Katic I, Xu L, Ciosk R.
G3 (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 10.1534/g3.115.019273.
PubMed
Associated Plasmids |
| Polymerase Theta is a key driver of genome evolution and of CRISPR/Cas9-mediated mutagenesis. van Schendel R, Roerink SF, Portegijs V, van den Heuvel S, Tijsterman M. Nat Commun. 2015 Jun 16;6:7394. doi: 10.1038/ncomms8394. PubMed |
| Context-dependent modulation of Pol II CTD phosphatase SSUP-72 regulates alternative polyadenylation in neuronal development. Chen F, Zhou Y, Qi YB, Khivansara V, Li H, Chun SY, Kim JK, Fu XD, Jin Y. Genes Dev. 2015 Nov 15;29(22):2377-90. doi: 10.1101/gad.266650.115. PubMed |
| The Stress Granule RNA-Binding Protein TIAR-1 Protects Female Germ Cells from Heat Shock in Caenorhabditis elegans. Huelgas-Morales G, Silva-Garcia CG, Salinas LS, Greenstein D, Navarro RE. G3 (Bethesda). 2016 Apr 7;6(4):1031-47. doi: 10.1534/g3.115.026815. PubMed |
| Neuroendocrine Coordination of Mitochondrial Stress Signaling and Proteostasis. Berendzen KM, Durieux J, Shao LW, Tian Y, Kim HE, Wolff S, Liu Y, Dillin A. Cell. 2016 Sep 8;166(6):1553-1563.e10. doi: 10.1016/j.cell.2016.08.042. PubMed |
| Engineering new balancer chromosomes in C. elegans via CRISPR/Cas9. Iwata S, Yoshina S, Suehiro Y, Hori S, Mitani S. Sci Rep. 2016 Sep 21;6:33840. doi: 10.1038/srep33840. PubMed |
| The tubulin repertoire of C. elegans sensory neurons and its context-dependent role in process outgrowth. Lockhead D, Schwarz EM, O'Hagan R, Bellotti S, Krieg M, Barr MM, Dunn AR, Sternberg PW, Goodman MB. Mol Biol Cell. 2016 Sep 21. pii: mbc.E16-06-0473. PubMed |
| Nuclei migrate through constricted spaces using microtubule motors and actin networks in C. elegans hypodermal cells. Bone CR, Chang YT, Cain NE, Murphy SP, Starr DA. Development. 2016 Nov 15;143(22):4193-4202. Epub 2016 Oct 3. PubMed |
| Neuropeptide signals cell non-autonomous mitochondrial unfolded protein response. Shao LW, Niu R, Liu Y. Cell Res. 2016 Nov;26(11):1182-1196. doi: 10.1038/cr.2016.118. Epub 2016 Oct 21. PubMed |
| FOS-1 functions as a transcriptional activator downstream of the C. elegans JNK homolog KGB-1. Zhang Z, Liu L, Twumasi-Boateng K, Block DH, Shapira M. Cell Signal. 2017 Jan;30:1-8. doi: 10.1016/j.cellsig.2016.11.010. Epub 2016 Nov 15. PubMed |
| PIGN prevents protein aggregation in the endoplasmic reticulum independently of its function in the GPI synthesis. Ihara S, Nakayama S, Murakami Y, Suzuki E, Asakawa M, Kinoshita T, Sawa H. J Cell Sci. 2016 Dec 15. pii: jcs.196717. PubMed |
| Analysis of postcytokinetic roles of cytokinetic components in Caenorhabditis elegans. Chai Y, Tian D, Li W, Ou G. Methods Cell Biol. 2017;137:253-266. doi: 10.1016/bs.mcb.2016.03.040. Epub 2016 Jun 1. PubMed |
| Tissue-specific regulation of alternative polyadenylation represses expression of a neuronal ankyrin isoform in C. elegans epidermal development. Chen F, Chisholm AD, Jin Y. Development. 2017 Feb 15;144(4):698-707. doi: 10.1242/dev.146001. Epub 2017 Jan 13. PubMed |
| Antidromic-rectifying gap junctions amplify chemical transmission at functionally mixed electrical-chemical synapses. Liu P, Chen B, Mailler R, Wang ZW. Nat Commun. 2017 Mar 20;8:14818. doi: 10.1038/ncomms14818. PubMed |
| Plastin increases cortical connectivity to facilitate robust polarization and timely cytokinesis. Ding WY, Ong HT, Hara Y, Wongsantichon J, Toyama Y, Robinson RC, Nedelec F, Zaidel-Bar R. J Cell Biol. 2017 May 1;216(5):1371-1386. doi: 10.1083/jcb.201603070. Epub 2017 Apr 11. PubMed |
| Twitchin kinase inhibits muscle activity. Matsunaga Y, Hwang H, Franke B, Williams R, Penley M, Qadota H, Yi H, Morran LT, Lu H, Mayans O, Benian GM. Mol Biol Cell. 2017 Jun 15;28(12):1591-1600. doi: 10.1091/mbc.E16-10-0707. Epub 2017 Apr 20. PubMed |
| RNA Binding Protein Vigilin Collaborates with miRNAs To Regulate Gene Expression for Caenorhabditis elegans Larval Development. Zabinsky RA, Weum B, Cui M, Han M. G3 (Bethesda). 2017 Jun 2. pii: g3.117.043414. doi: 10.1534/g3.117.043414. PubMed |
| Genome Editing of C. elegans. Sugi T. Methods Mol Biol. 2017;1630:247-254. doi: 10.1007/978-1-4939-7128-2_20. PubMed |
| Mechanism of biofilm-mediated stress resistance and lifespan extension in C. elegans. Smolentseva O, Gusarov I, Gautier L, Shamovsky I, DeFrancesco AS, Losick R, Nudler E. Sci Rep. 2017 Aug 2;7(1):7137. doi: 10.1038/s41598-017-07222-8. PubMed |
| Targeted genome editing in Caenorhabditis elegans using CRISPR/Cas9. Farboud B. Wiley Interdiscip Rev Dev Biol. 2017 Nov;6(6). doi: 10.1002/wdev.287. Epub 2017 Aug 15. PubMed |
| BORC Regulates the Axonal Transport of Synaptic Vesicle Precursors by Activating ARL-8. Niwa S, Tao L, Lu SY, Liew GM, Feng W, Nachury MV, Shen K. Curr Biol. 2017 Sep 11;27(17):2569-2578.e4. doi: 10.1016/j.cub.2017.07.013. Epub 2017 Aug 17. PubMed |
| LET-413/Erbin acts as a RAB-5 effector to promote RAB-10 activation during endocytic recycling. Liu H, Wang S, Hang W, Gao J, Zhang W, Cheng Z, Yang C, He J, Zhou J, Chen J, Shi A. J Cell Biol. 2018 Jan 2;217(1):299-314. doi: 10.1083/jcb.201705136. Epub 2017 Oct 27. PubMed |
| An Intracellular Pathogen Response Pathway Promotes Proteostasis in C. elegans. Reddy KC, Dror T, Sowa JN, Panek J, Chen K, Lim ES, Wang D, Troemel ER. Curr Biol. 2017 Nov 20;27(22):3544-3553.e5. doi: 10.1016/j.cub.2017.10.009. Epub 2017 Nov 2. PubMed |
| Polo-like Kinase Couples Cytoplasmic Protein Gradients in the C. elegans Zygote. Han B, Antkowiak KR, Fan X, Rutigliano M, Ryder SP, Griffin EE. Curr Biol. 2018 Jan 8;28(1):60-69.e8. doi: 10.1016/j.cub.2017.11.048. Epub 2017 Dec 21. PubMed |
| Tumor suppressor APC is an attenuator of spindle-pulling forces during C. elegans asymmetric cell division. Sugioka K, Fielmich LE, Mizumoto K, Bowerman B, van den Heuvel S, Kimura A, Sawa H. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):E954-E963. doi: 10.1073/pnas.1712052115. Epub 2018 Jan 18. PubMed |
| A Neuronal piRNA Pathway Inhibits Axon Regeneration in C. elegans. Kim KW, Tang NH, Andrusiak MG, Wu Z, Chisholm AD, Jin Y. Neuron. 2018 Feb 7;97(3):511-519.e6. doi: 10.1016/j.neuron.2018.01.014. Epub 2018 Jan 27. PubMed |
| TMC Proteins Modulate Egg Laying and Membrane Excitability through a Background Leak Conductance in C. elegans. Yue X, Zhao J, Li X, Fan Y, Duan D, Zhang X, Zou W, Sheng Y, Zhang T, Yang Q, Luo J, Duan S, Xiao R, Kang L. Neuron. 2018 Feb 7;97(3):571-585.e5. doi: 10.1016/j.neuron.2017.12.041. Epub 2018 Jan 27. PubMed |
| Genetic Suppression of Basement Membrane Defects in Caenorhabditis elegans by Gain of Function in Extracellular Matrix and Cell-Matrix Attachment Genes. Gotenstein JR, Koo CC, Ho TW, Chisholm AD. Genetics. 2018 Apr;208(4):1499-1512. doi: 10.1534/genetics.118.300731. Epub 2018 Feb 12. PubMed |
| MIB-1 Is Required for Spermatogenesis and Facilitates LIN-12 and GLP-1 Activity in Caenorhabditis elegans. Ratliff M, Hill-Harfe KL, Gleason EJ, Ling H, Kroft TL, L'Hernault SW. Genetics. 2018 May;209(1):173-193. doi: 10.1534/genetics.118.300807. Epub 2018 Mar 12. PubMed |
| SAC-1 ensures epithelial endocytic recycling by restricting ARF-6 activity. Chen D, Yang C, Liu S, Hang W, Wang X, Chen J, Shi A. J Cell Biol. 2018 Jun 4;217(6):2121-2139. doi: 10.1083/jcb.201711065. Epub 2018 Mar 21. PubMed |
| The kinases male germ cell-associated kinase and cell cycle-related kinase regulate kinesin-2 motility in Caenorhabditis elegans neuronal cilia. Yi P, Xie C, Ou G. Traffic. 2018 Jul;19(7):522-535. doi: 10.1111/tra.12572. Epub 2018 May 17. PubMed |
| The cisd gene family regulates physiological germline apoptosis through ced-13 and the canonical cell death pathway in Caenorhabditis elegans. King SD, Gray CF, Song L, Nechushtai R, Gumienny TL, Mittler R, Padilla PA. Cell Death Differ. 2019 Jan;26(1):162-178. doi: 10.1038/s41418-018-0108-5. Epub 2018 Apr 17. PubMed |
Streptothricin acetyl transferase 2 (Sat2): A dominant selection marker for Caenorhabditis elegans genome editing.
Obinata H, Sugimoto A, Niwa S.
PLoS One. 2018 May 9;13(5):e0197128. doi: 10.1371/journal.pone.0197128. eCollection 2018.
PubMed
Associated Plasmids |
| The zinc transporter ZIPT-7.1 regulates sperm activation in nematodes. Zhao Y, Tan CH, Krauchunas A, Scharf A, Dietrich N, Warnhoff K, Yuan Z, Druzhinina M, Gu SG, Miao L, Singson A, Ellis RE, Kornfeld K. PLoS Biol. 2018 Jun 7;16(6):e2005069. doi: 10.1371/journal.pbio.2005069. eCollection 2018 Jun. PubMed |
Highly efficient RNAi and Cas9-based auto-cloning systems for C. elegans research.
Sturm A, Saskoi E, Tibor K, Weinhardt N, Vellai T.
Nucleic Acids Res. 2018 Jun 19. pii: 5040046. doi: 10.1093/nar/gky516.
PubMed
Associated Plasmids |
OFF-responses of interneurons optimize avoidance behaviors depending on stimulus strength via electrical synapses.
Hori S, Oda S, Suehiro Y, Iino Y, Mitani S.
PLoS Genet. 2018 Jun 25;14(6):e1007477. doi: 10.1371/journal.pgen.1007477. eCollection 2018 Jun.
PubMed
Associated Plasmids |
| Conserved SUN-KASH Interfaces Mediate LINC Complex-Dependent Nuclear Movement and Positioning. Cain NE, Jahed Z, Schoenhofen A, Valdez VA, Elkin B, Hao H, Harris NJ, Herrera LA, Woolums BM, Mofrad MRK, Luxton GWG, Starr DA. Curr Biol. 2018 Oct 8;28(19):3086-3097.e4. doi: 10.1016/j.cub.2018.08.001. Epub 2018 Sep 20. PubMed |
| CRELD1 is an evolutionarily-conserved maturational enhancer of ionotropic acetylcholine receptors. D'Alessandro M, Richard M, Stigloher C, Gache V, Boulin T, Richmond JE, Bessereau JL. Elife. 2018 Nov 7;7. pii: 39649. doi: 10.7554/eLife.39649. PubMed |
| Syncytial germline architecture is actively maintained by contraction of an internal actomyosin corset. Priti A, Ong HT, Toyama Y, Padmanabhan A, Dasgupta S, Krajnc M, Zaidel-Bar R. Nat Commun. 2018 Nov 8;9(1):4694. doi: 10.1038/s41467-018-07149-2. PubMed |
| Regulation of germ cell development by ARI1 family ubiquitin ligases in C. elegans. Poush JA, Blouin NA, Di Bona KR, Lazetic V, Fay DS. Sci Rep. 2018 Dec 10;8(1):17737. doi: 10.1038/s41598-018-35691-y. PubMed |
| Adhesive L1CAM-Robo Signaling Aligns Growth Cone F-Actin Dynamics to Promote Axon-Dendrite Fasciculation in C. elegans. Chen CH, Hsu HW, Chang YH, Pan CL. Dev Cell. 2019 Jan 28;48(2):215-228.e5. doi: 10.1016/j.devcel.2018.10.028. Epub 2018 Dec 13. PubMed |
| Systems Properties and Spatiotemporal Regulation of Cell Position Variability during Embryogenesis. Li X, Zhao Z, Xu W, Fan R, Xiao L, Ma X, Du Z. Cell Rep. 2019 Jan 8;26(2):313-321.e7. doi: 10.1016/j.celrep.2018.12.052. PubMed |
| SUMO peptidase ULP-4 regulates mitochondrial UPR-mediated innate immunity and lifespan extension. Gao K, Li Y, Hu S, Liu Y. Elife. 2019 Jan 15;8. pii: 41792. doi: 10.7554/eLife.41792. PubMed |
| Construction of a germline-specific RNAi tool in C. elegans. Zou L, Wu D, Zang X, Wang Z, Wu Z, Chen D. Sci Rep. 2019 Feb 20;9(1):2354. doi: 10.1038/s41598-019-38950-8. PubMed |
| Ectopic Germ Cells Can Induce Niche-like Enwrapment by Neighboring Body Wall Muscle. Gordon KL, Payne SG, Linden-High LM, Pani AM, Goldstein B, Hubbard EJA, Sherwood DR. Curr Biol. 2019 Mar 4;29(5):823-833.e5. doi: 10.1016/j.cub.2019.01.056. Epub 2019 Feb 21. PubMed |
| A microRNA switch controls dietary restriction-induced longevity through Wnt signaling. Xu Y, He Z, Song M, Zhou Y, Shen Y. EMBO Rep. 2019 May;20(5). pii: embr.201846888. doi: 10.15252/embr.201846888. Epub 2019 Mar 14. PubMed |
NATF (Native And Tissue-Specific Fluorescence): A Strategy for Bright, Tissue-Specific GFP Labeling of Native Proteins in Caenorhabditis elegans.
He S, Cuentas-Condori A, Miller DM.
Genetics. 2019 Apr 5. pii: genetics.119.302063. doi: 10.1534/genetics.119.302063.
PubMed
Associated Plasmids |
| A Model of Hereditary Sensory and Autonomic Neuropathy Type 1 Reveals a Role of Glycosphingolipids in Neuronal Polarity. Cui M, Ying R, Jiang X, Li G, Zhang X, Zheng J, Tam KY, Liang B, Shi A, Gobel V, Zhang H. J Neurosci. 2019 Jul 17;39(29):5816-5834. doi: 10.1523/JNEUROSCI.2541-18.2019. Epub 2019 May 28. PubMed |
| Spectrin-based membrane skeleton supports ciliogenesis. Jia R, Li D, Li M, Chai Y, Liu Y, Xie Z, Shao W, Xie C, Li L, Huang X, Chen L, Li W, Ou G. PLoS Biol. 2019 Jul 12;17(7):e3000369. doi: 10.1371/journal.pbio.3000369. eCollection 2019 Jul. PubMed |
| DAF-16/FOXO promotes taste avoidance learning independently of axonal insulin-like signaling. Nagashima T, Iino Y, Tomioka M. PLoS Genet. 2019 Jul 19;15(7):e1008297. doi: 10.1371/journal.pgen.1008297. eCollection 2019 Jul. PubMed |
| Translational Regulation of Non-autonomous Mitochondrial Stress Response Promotes Longevity. Lan J, Rollins JA, Zang X, Wu D, Zou L, Wang Z, Ye C, Wu Z, Kapahi P, Rogers AN, Chen D. Cell Rep. 2019 Jul 23;28(4):1050-1062.e6. doi: 10.1016/j.celrep.2019.06.078. PubMed |
Disease-associated mutations hyperactivate KIF1A motility and anterograde axonal transport of synaptic vesicle precursors.
Chiba K, Takahashi H, Chen M, Obinata H, Arai S, Hashimoto K, Oda T, McKenney RJ, Niwa S.
Proc Natl Acad Sci U S A. 2019 Aug 27. pii: 1905690116. doi: 10.1073/pnas.1905690116.
PubMed
Associated Plasmids |
| Gene silencing by double-stranded RNA from C. elegans neurons reveals functional mosaicism of RNA interference. Ravikumar S, Devanapally S, Jose AM. Nucleic Acids Res. 2019 Nov 4;47(19):10059-10071. doi: 10.1093/nar/gkz748. PubMed |
| A secreted microRNA disrupts autophagy in distinct tissues of Caenorhabditis elegans upon ageing. Zhou Y, Wang X, Song M, He Z, Cui G, Peng G, Dieterich C, Antebi A, Jing N, Shen Y. Nat Commun. 2019 Oct 23;10(1):4827. doi: 10.1038/s41467-019-12821-2. PubMed |
| henn-1/HEN1 Promotes Germline Immortality in Caenorhabditis elegans. Svendsen JM, Reed KJ, Vijayasarathy T, Montgomery BE, Tucci RM, Brown KC, Marks TN, Nguyen DAH, Phillips CM, Montgomery TA. Cell Rep. 2019 Dec 3;29(10):3187-3199.e4. doi: 10.1016/j.celrep.2019.10.114. PubMed |
| Epidermal Growth Factor Signaling Promotes Sleep through a Combined Series and Parallel Neural Circuit. Konietzka J, Fritz M, Spiri S, McWhirter R, Leha A, Palumbos S, Costa WS, Oranth A, Gottschalk A, Miller DM 3rd, Hajnal A, Bringmann H. Curr Biol. 2020 Jan 6;30(1):1-16.e13. doi: 10.1016/j.cub.2019.10.048. Epub 2019 Dec 12. PubMed |
| Epidermal control of axonal attachment via beta-spectrin and the GTPase-activating protein TBC-10 prevents axonal degeneration. Coakley S, Ritchie FK, Galbraith KM, Hilliard MA. Nat Commun. 2020 Jan 9;11(1):133. doi: 10.1038/s41467-019-13795-x. PubMed |
| Distinct roles of two eIF4E isoforms in the germline of Caenorhabditis elegans. Huggins HP, Subash JS, Stoffel H, Henderson MA, Hoffman JL, Buckner DS, Sengupta MS, Boag PR, Lee MH, Keiper BD. J Cell Sci. 2020 Mar 30;133(6). pii: jcs.237990. doi: 10.1242/jcs.237990. PubMed |
| Slo2 potassium channel function depends on RNA editing-regulated expression of a SCYL1 protein. Niu LG, Liu P, Wang ZW, Chen B. Elife. 2020 Apr 21;9. pii: 53986. doi: 10.7554/eLife.53986. PubMed |
| Translesion synthesis polymerases are dispensable for C. elegans reproduction but suppress genome scarring by polymerase theta-mediated end joining. van Bostelen I, van Schendel R, Romeijn R, Tijsterman M. PLoS Genet. 2020 Apr 24;16(4):e1008759. doi: 10.1371/journal.pgen.1008759. eCollection 2020 Apr. PubMed |
| Optimal sidestepping of intraflagellar transport kinesins regulates structure and function of sensory cilia. Xie C, Li L, Li M, Shao W, Zuo Q, Huang X, Chen R, Li W, Brunnbauer M, Okten Z, Chen L, Ou G. EMBO J. 2020 Jun 17;39(12):e103955. doi: 10.15252/embj.2019103955. Epub 2020 Apr 27. PubMed |
| Modified auxin improves the auxin-inducible degradation (AID) system for laid C. elegans embryos. Negishi T, Asakawa M, Kanemaki M, Sawa H. MicroPubl Biol. 2019 Dec 10;2019. doi: 10.17912/micropub.biology.000190. PubMed |
| New allele of C. elegans gene pign-1, named as xyz11. Narimatsu T, Ihara S. MicroPubl Biol. 2019 Jan 18;2019. doi: 10.17912/micropub.biology.000088. PubMed |
| The spectrin-based membrane skeleton is asymmetric and remodels during neural development in C. elegans. Jia R, Chai Y, Xie C, Liu G, Zhu Z, Huang K, Li W, Ou G. J Cell Sci. 2020 Aug 5;133(15). pii: jcs.248583. doi: 10.1242/jcs.248583. PubMed |
| Caenorhabditis elegans Flamingo FMI-1 controls dendrite self-avoidance through F-actin assembly. Hsu HW, Liao CP, Chiang YC, Syu RT, Pan CL. Development. 2020 Jul 31;147(14). pii: dev.179168. doi: 10.1242/dev.179168. PubMed |
| Molecular basis of junctional current rectification at an electrical synapse. Shui Y, Liu P, Zhan H, Chen B, Wang ZW. Sci Adv. 2020 Jul 3;6(27):eabb3076. doi: 10.1126/sciadv.abb3076. eCollection 2020 Jul. PubMed |
| The Conserved ASCL1/MASH-1 Ortholog HLH-3 Specifies Sex-Specific Ventral Cord Motor Neuron Fate in Caenorhabditis elegans. Perez LM, Alfonso A. G3 (Bethesda). 2020 Nov 5;10(11):4201-4213. doi: 10.1534/g3.120.401458. PubMed |
| IGLR-2, a Leucine-Rich Repeat Domain Containing Protein, Is Required for the Host Defense in Caenorhabditis elegans. Kuo CJ, Hsu YC, Wang ST, Liou BY, Lim SB, Chen YW, Chen CS. Front Immunol. 2020 Nov 30;11:561337. doi: 10.3389/fimmu.2020.561337. eCollection 2020. PubMed |
| The mitochondrial localized CISD-3.1/CISD-3.2 proteins are required to maintain normal germline structure and function in Caenorhabditis elegans. King SD, Gray CF, Song L, Mittler R, Padilla PA. PLoS One. 2021 Feb 5;16(2):e0245174. doi: 10.1371/journal.pone.0245174. eCollection 2021. PubMed |
| Intertissue small RNA communication mediates the acquisition and inheritance of hormesis in Caenorhabditis elegans. Okabe E, Uno M, Kishimoto S, Nishida E. Commun Biol. 2021 Feb 16;4(1):207. doi: 10.1038/s42003-021-01692-3. PubMed |
| The decrease of intraflagellar transport impairs sensory perception and metabolism in ageing. Zhang Y, Zhang X, Dai Y, Song M, Zhou Y, Zhou J, Yan X, Shen Y. Nat Commun. 2021 Mar 19;12(1):1789. doi: 10.1038/s41467-021-22065-8. PubMed |
| N-terminally endogenous mKate2-tagged HIM-4 in Caenorhabditis elegans. Ihara S. MicroPubl Biol. 2021 Apr 29;2021. doi: 10.17912/micropub.biology.000386. PubMed |
| Expression Patterns and Levels of All Tubulin Isotypes Analyzed in GFP Knock-In C. elegans Strains. Nishida K, Tsuchiya K, Obinata H, Onodera S, Honda Y, Lai YC, Haruta N, Sugimoto A. Cell Struct Funct. 2021 Jun 30;46(1):51-64. doi: 10.1247/csf.21022. Epub 2021 May 8. PubMed |
| Preservation of lagging strand integrity at sites of stalled replication by Pol alpha-primase and 9-1-1 complex. van Schendel R, Romeijn R, Buijs H, Tijsterman M. Sci Adv. 2021 May 19;7(21). pii: 7/21/eabf2278. doi: 10.1126/sciadv.abf2278. Print 2021 May. PubMed |
| Insulin signaling regulates longevity through protein phosphorylation in Caenorhabditis elegans. Li WJ, Wang CW, Tao L, Yan YH, Zhang MJ, Liu ZX, Li YX, Zhao HQ, Li XM, He XD, Xue Y, Dong MQ. Nat Commun. 2021 Jul 27;12(1):4568. doi: 10.1038/s41467-021-24816-z. PubMed |
| Two isoforms of the essential C. elegans Argonaute CSR-1 differentially regulate sperm and oocyte fertility. Charlesworth AG, Seroussi U, Lehrbach NJ, Renaud MS, Sundby AE, Molnar RI, Lao RX, Willis AR, Woock JR, Aber MJ, Diao AJ, Reinke AW, Ruvkun G, Claycomb JM. Nucleic Acids Res. 2021 Sep 7;49(15):8836-8865. doi: 10.1093/nar/gkab619. PubMed |
| Antisense ribosomal siRNAs inhibit RNA polymerase I-directed transcription in C. elegans. Liao S, Chen X, Xu T, Jin Q, Xu Z, Xu D, Zhou X, Zhu C, Guang S, Feng X. Nucleic Acids Res. 2021 Sep 20;49(16):9194-9210. doi: 10.1093/nar/gkab662. PubMed |
| Actin filament debranching regulates cell polarity during cell migration and asymmetric cell division. Xie C, Jiang Y, Zhu Z, Huang S, Li W, Ou G. Proc Natl Acad Sci U S A. 2021 Sep 14;118(37). pii: 2100805118. doi: 10.1073/pnas.2100805118. PubMed |
| Reduction of Derlin activity suppresses Notch-dependent tumours in the C. elegans germ line. Singh R, Smit RB, Wang X, Wang C, Racher H, Hansen D. PLoS Genet. 2021 Sep 23;17(9):e1009687. doi: 10.1371/journal.pgen.1009687. eCollection 2021 Sep. PubMed |
| Mechanistic insights into central spindle assembly mediated by the centralspindlin complex. Pan H, Guan R, Zhao R, Ou G, Chen Z. Proc Natl Acad Sci U S A. 2021 Oct 5;118(40). pii: 2112039118. doi: 10.1073/pnas.2112039118. PubMed |
| PDZD-8 and TEX-2 regulate endosomal PI(4,5)P2 homeostasis via lipid transport to promote embryogenesis in C. elegans. Jeyasimman D, Ercan B, Dharmawan D, Naito T, Sun J, Saheki Y. Nat Commun. 2021 Oct 18;12(1):6065. doi: 10.1038/s41467-021-26177-z. PubMed |
| A somatic proteoglycan controls Notch-directed germ cell fate. Gopal S, Amran A, Elton A, Ng L, Pocock R. Nat Commun. 2021 Nov 18;12(1):6708. doi: 10.1038/s41467-021-27039-4. PubMed |
The auxin-inducible degron 2 (AID2) system enables controlled protein knockdown during embryogenesis and development in Caenorhabditis elegans.
Negishi T, Kitagawa S, Horii N, Tanaka Y, Haruta N, Sugimoto A, Sawa H, Hayashi KI, Harata M, Kanemaki MT.
Genetics. 2021 Dec 2. pii: 6448232. doi: 10.1093/genetics/iyab218.
PubMed
Associated Plasmids |
| Helicase Q promotes homology-driven DNA double-strand break repair and prevents tandem duplications. Kamp JA, Lemmens BBLG, Romeijn RJ, Changoer SC, van Schendel R, Tijsterman M. Nat Commun. 2021 Dec 8;12(1):7126. doi: 10.1038/s41467-021-27408-z. PubMed |
| AP-1 Recruits SMAP-1/SMAPs to the trans-Golgi Network to Promote Sorting in Polarized Epithelia. Wang S, Yao L, Zhang W, Cheng Z, Hu C, Liu H, Yan Y, Shi A. Front Cell Dev Biol. 2021 Nov 25;9:774401. doi: 10.3389/fcell.2021.774401. eCollection 2021. PubMed |
| Membrane-associated cytoplasmic granules carrying the Argonaute protein WAGO-3 enable paternal epigenetic inheritance in Caenorhabditis elegans. Schreier J, Dietz S, Boermel M, Oorschot V, Seistrup AS, de Jesus Domingues AM, Bronkhorst AW, Nguyen DAH, Phillis S, Gleason EJ, L'Hernault SW, Phillips CM, Butter F, Ketting RF. Nat Cell Biol. 2022 Feb;24(2):217-229. doi: 10.1038/s41556-021-00827-2. Epub 2022 Feb 7. PubMed |
| Hox proteins interact to pattern neuronal subtypes in Caenorhabditis elegans males. Kalis AK, Sterrett MC, Armstrong C, Ballmer A, Burkstrand K, Chilson E, Emlen E, Ferrer E, Loeb S, Olin T, Tran K, Wheeler A, Ross Wolff J. Genetics. 2022 Apr 4;220(4):iyac010. doi: 10.1093/genetics/iyac010. PubMed |
| Post-insemination selection dominates pre-insemination selection in driving rapid evolution of male competitive ability. Kasimatis KR, Moerdyk-Schauwecker MJ, Lancaster R, Smith A, Willis JH, Phillips PC. PLoS Genet. 2022 Feb 14;18(2):e1010063. doi: 10.1371/journal.pgen.1010063. eCollection 2022 Feb. PubMed |
| Small RNAs couple embryonic developmental programs to gut microbes. Ohno H, Bao Z. Sci Adv. 2022 Mar 25;8(12):eabl7663. doi: 10.1126/sciadv.abl7663. Epub 2022 Mar 23. PubMed |
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