pSLIK MEK5-DD-mRFP1 neo
(Plasmid #47548)

• Purpose: Lentiviral expression vector for an inducible mRFP1-tagged constitutively active MEK5 mutant
• Depositing Lab: Kevin Janes
• Publication: Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-76. doi: 10.1016/j.chom.2012.11.009. Epub 2013 Jan 16.

Backbone

• Vector backbone: pSLIK NEO
• Backbone manufacturer: Addgene #25735
• Backbone size w/o insert (bp): 13286
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MEK5-DD-mRFP1
• Alt name: MEK5
• Alt name: MAP2K5
• Alt name: MAPKK5
• Species: H. sapiens (human)
• Insert Size (bp): 2025
• Mutation: S311D, T315D
• Entrez Gene: MAP2K5 (a.k.a. HsT17454, MAPKK5, MEK5, PRKMK5)
• Tag / Fusion Protein:
  • mRFP (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hUBCpro-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene #23805
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry