pEN_TT 3xHA-MKK6-EE
(Plasmid
#47544)
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PurposeGateway entry vector for an inducible 3xHA-tagged constitutively active MKK6 mutant
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 47544 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEN_TTmiRc2
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Backbone manufacturerAddgene #2752
- Backbone size w/o insert (bp) 4422
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Vector typeEntry vector
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name3xHA-MKK6-EE
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Alt nameMKK6
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Alt namePRMK6
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Alt nameMEK6
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SpeciesH. sapiens (human)
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Insert Size (bp)1095
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MutationS207E, T211E
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GenBank IDNM_002758
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Entrez GeneMAP2K6 (a.k.a. MAPKK6, MEK6, MKK6, PRKMK6, SAPKK-3, SAPKK3)
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Tag
/ Fusion Protein
- 3XHA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site MfeI (not destroyed)
- 5′ sequencing primer pCEP-fwd
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAddgene #13518
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEN_TT 3xHA-MKK6-EE was a gift from Kevin Janes (Addgene plasmid # 47544 ; http://n2t.net/addgene:47544 ; RRID:Addgene_47544) -
For your References section:
An ERK-p38 subnetwork coordinates host cell apoptosis and necrosis during coxsackievirus B3 infection. Jensen KJ, Garmaroudi FS, Zhang J, Lin J, Boroomand S, Zhang M, Luo Z, Yang D, Luo H, McManus BM, Janes KA. Cell Host Microbe. 2013 Jan 16;13(1):67-76. doi: 10.1016/j.chom.2012.11.009. Epub 2013 Jan 16. 10.1016/j.chom.2012.11.009 PubMed 23332156