pMSCVpuroATT
(Plasmid #47539)

• Purpose: (Empty Backbone) Retroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)
• Depositing Lab: David Weinstock
• Publication: Shindoh et al PLoS One. 2012;7(11):e49201. doi: 10.1371/journal.pone.0049201. Epub 2012 Nov 7.

Backbone

• Vector backbone: pMSCVpuro
• Backbone manufacturer: Clontech
• Backbone size (bp): 6300
• Modifications to backbone: Inserted Reading Frame Cassette A into the retroviral expression vector pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: Unknown

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: pLXSN-5
• 3′ sequencing primer: MSCV-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry