pMSCVpuroATT
(Plasmid
#47539)
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Purpose(Empty Backbone) Retroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47539 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMSCVpuro
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Backbone manufacturerClontech
- Backbone size (bp) 6300
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Modifications to backboneInserted Reading Frame Cassette A into the retroviral expression vector pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberUnknown
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pLXSN-5
- 3′ sequencing primer MSCV-rev (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCVpuroATT was a gift from David Weinstock (Addgene plasmid # 47539 ; http://n2t.net/addgene:47539 ; RRID:Addgene_47539) -
For your References section:
Next-generation cDNA screening for oncogene and resistance phenotypes. Shindoh N, Yoda A, Yoda Y, Sullivan TJ, Weigert O, Lane AA, Kopp N, Bird L, Rodig SJ, Fox EA, Weinstock DM. PLoS One. 2012;7(11):e49201. doi: 10.1371/journal.pone.0049201. Epub 2012 Nov 7. 10.1371/journal.pone.0049201 PubMed 23145123