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Addgene

pcDNA3 P-cad
(Plasmid #47502)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47502 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cadherin 3, type 1, P-cadherin (placental)
  • Alt name
    P-cad
  • Alt name
    CDH3
  • Alt name
    CDHP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2500
  • GenBank ID
    1001 NM_001793
  • Entrez Gene
    CDH3 (a.k.a. CDHP, HJMD, PCAD)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 P-cad was a gift from Alice Wong (Addgene plasmid # 47502 ; http://n2t.net/addgene:47502 ; RRID:Addgene_47502)

  • For your References section:

    Cadherin switching and activation of p120 catenin signaling are mediators of gonadotropin-releasing hormone to promote tumor cell migration and invasion in ovarian cancer. Cheung LW, Leung PC, Wong AS. Oncogene. 2010 Apr 22;29(16):2427-40. doi: 10.1038/onc.2009.523. Epub 2010 Feb 1. 10.1038/onc.2009.523 PubMed 20118984
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