LITE2.0_pAAV_hSyn_TALE(Grm2)-GS-CIB1(NLS*,∆318-334)_WPRE_bGHpA
(Plasmid #47456)

• Purpose: LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Grm2. Synapsin promoter for neuronal expression.
• Depositing Lab: Feng Zhang
• Publication: Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature12466.

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Backbone

• Vector backbone: pAAV_hSyn_WPRE_bGHpA
• Backbone manufacturer: Zhang Lab
• Backbone size w/o insert (bp): 4280
• Total vector size (bp): 7148
• Vector type: Mammalian Expression, AAV ; TALE

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TALE(Grm2)-GS-CIB1(NLS*,∆318-334)
• Species: Synthetic
• Insert Size (bp): 2868
• Promoter: hSyn human synapsin (mammalian neuronal expression)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: multiple

Resource Information

• Supplemental Documents:
  • LITE2.0 pAAV_hSyn_TALE(Grm2)-GS-CIB1(NLS-,∆318-334)_WPRE_bGHpA.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry