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LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA
(Plasmid #47455)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47455 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV_hSyn_WPRE_bGHpA
  • Backbone manufacturer
    Zhang Lab
  • Backbone size w/o insert (bp) 4280
  • Total vector size (bp) 6818
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLS(alpha-imp)-CRY2PHR-NLS-VP64
  • Species
    Synthetic
  • Insert Size (bp)
    2538
  • Promoter hSyn human synapsin (mammalian neuronal expression)
  • Tag / Fusion Protein
    • 2A_GFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA was a gift from Feng Zhang (Addgene plasmid # 47455 ; http://n2t.net/addgene:47455 ; RRID:Addgene_47455)

  • For your References section:

    Optical control of mammalian endogenous transcription and epigenetic states. Konermann S, Brigham MD, Trevino A, Hsu PD, Heidenreich M, Le Cong, Platt RJ, Scott DA, Church GM, Zhang F. Nature. 2013 Jul 23. doi: 10.1038/nature12466. 10.1038/nature12466 PubMed 23877069
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