Flag-Jun4A-Myc
(Plasmid #47444)

• Purpose: mammalian expression of mutant c-Jun (four N-terminal residues phosphorylated by JNK are mutated into Alanines)
• Depositing Lab: Axel Behrens
• Publication: Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi: 10.1038/nature09607. Epub 2011 Jan 2.

Backbone

• Vector backbone: pCMV-Tag2A
• Backbone size w/o insert (bp): 4300
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Jun 4A mutant
• Alt name: Junc
• Alt name: c-Jun
• Alt name: AP-1
• Species: M. musculus (mouse)
• Mutation: four N-terminal residues phosphorylated by JNK, serines 63 and 73, and threonines 91 and 93, are mutated into alanines
• Entrez Gene: Jun (a.k.a. AP-1, Junc, c-jun)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Flag (N terminal on backbone)
  • myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry