pGEM-mcpGFP
(Plasmid #47442)

• Purpose: contains modified circularly permutated EGFP (mcpGFP)
• Depositing Lab: Wolf Frommer
• Publication: De Michele et al eLife. 2013 Jul 2;2:e00800.

Backbone

• Vector backbone: pGEM T-easy
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 3700
• Modifications to backbone: there are some residual attB1 and attB2 nucleotides in the linkers between pGEM and the insert.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mcpGFP
• Alt name: mutated circular permuted EGFP
• Species: Synthetic
• Insert Size (bp): 723
• Mutation: M154K, V164A, S176G, D181Y, T204V, A207K, V190I

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

same as
Tian L, Hires SA, Mao T, Huber D, Chiappe ME, Chalasani SH, et al. 2009. Imaging neural activity in worms, flies
and mice with improved GCaMP calcium indicators. Nat Methods 6:875–81. doi: 10.1038/nmeth.1398.