pJ251-GERC
(Plasmid #47441)

• Purpose: Expresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.
• Depositing Lab: George Church
• Publication: Kosuri pGERC reporter plasmid (unpublished)

Backbone

• Vector backbone: pJ251
• Backbone manufacturer: DNA2.0
• Backbone size w/o insert (bp): 2628
• Total vector size (bp): 4992
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: Can be grown at 37C as well.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Gene Expression Reporter Construct
• Alt name: mCherry
• Alt name: GFP
• Species: Synthetic; E. coli
• Insert Size (bp): 2364

Resource Information

• A portion of this plasmid was derived from a plasmid made by: De novo synthesis
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry