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pCDNA_B2AR-no-pep
(Plasmid #47439)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47439 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA5_FRT
  • Backbone size w/o insert (bp) 5070
  • Total vector size (bp) 8183
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    beta2-adrenergic receptor
  • Alt name
    ADRB2
  • Alt name
    beta2-AR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3181
  • GenBank ID
    NM_000024.5
  • Entrez Gene
    ADRB2 (a.k.a. ADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR)
  • Promoter pCMV
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • mCitrine (C terminal on insert)
    • 10 nm ER/K helix (C terminal on insert)
    • mCerulean (C terminal on insert)
    • no-pep (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer BGHR Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Notes:
(1) The pCDNA/FRT vector contains an extra XbaI site.
(2) The XbaI restriction enzyme is sensitive to dam methylation.
(3) No-pep sensor contains a short repeating GSG sequence at the C-terminus of mCerulean.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA_B2AR-no-pep was a gift from Sivaraj Sivaramakrishnan (Addgene plasmid # 47439 ; http://n2t.net/addgene:47439 ; RRID:Addgene_47439)

  • For your References section:

    Detection of G Protein-selective G Protein-coupled Receptor (GPCR) Conformations in Live Cells. Malik RU, Ritt M, Devree BT, Neubig RR, Sunahara RK, Sivaramakrishnan S. J Biol Chem. 2013 Jun 14;288(24):17167-78. doi: 10.1074/jbc.M113.464065. Epub 2013 Apr 29. 10.1074/jbc.M113.464065 PubMed 23629648
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