Flag-Tagged Clavesin1 (pCMV-Tag2B-Sec14a)
(Plasmid
#47430)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 47430 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV-Tag2B
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4324
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameClavesin1
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Alt nameCLVS1
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SpeciesH. sapiens (human)
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Entrez GeneCLVS1 (a.k.a. C6orf212L, CRALBPL, RLBP1L1)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Flag-Tagged Clavesin1 (pCMV-Tag2B-Sec14a) was a gift from Peter McPherson (Addgene plasmid # 47430 ; http://n2t.net/addgene:47430 ; RRID:Addgene_47430) -
For your References section:
The clavesin family, neuron-specific lipid- and clathrin-binding Sec14 proteins regulating lysosomal morphology. Katoh Y, Ritter B, Gaffry T, Blondeau F, Honing S, McPherson PS. J Biol Chem. 2009 Oct 2;284(40):27646-54. doi: 10.1074/jbc.M109.034884. Epub 2009 Aug 3. 10.1074/jbc.M109.034884 PubMed 19651769