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Addgene

GFP-Rab35 S22N inactive
(Plasmid #47426)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47426 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    clontech
  • Backbone size w/o insert (bp) 4731
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rab35 S22N
  • Alt name
    Rab35
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    600
  • Mutation
    S22N inactive
  • Entrez Gene
    RAB35 (a.k.a. H-ray, RAB1C, RAY)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-Rab35 S22N inactive was a gift from Peter McPherson (Addgene plasmid # 47426 ; http://n2t.net/addgene:47426 ; RRID:Addgene_47426)
  • For your References section:

    Interplay between Rab35 and Arf6 controls cargo recycling to coordinate cell adhesion and migration. Allaire PD, Seyed Sadr M, Chaineau M, Seyed Sadr E, Konefal S, Fotouhi M, Maret D, Ritter B, Del Maestro RF, McPherson PS. J Cell Sci. 2013 Feb 1;126(Pt 3):722-31. doi: 10.1242/jcs.112375. Epub 2012 Dec 21. 10.1242/jcs.112375 PubMed 23264734